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Enzymes de dépolymérisation d'exopolysaccharides bactériens marins ArchiMer
Lelchat, Florian.
Exopolysaccharides (EPSs) are a class of biopolymer synthesized by Eukarya, Archea and Procarya.Bacterial EPSs are involved in biofilm establishment and biofouling phenomenon. These polymers have physicochemical and biological properties suitable with biotechnological valorization. At the opposite, their involvment in biofouling of pathogenic strains can be problematic.Enzymatic depolymerization process are necessary for EPSs structural elucidation, Bioactive oligosaccharides production or to disrupt polysaccharidic biofilms. The highlight of enzymatic phenomenon can help to understand biogeochimical process in the ocean. Nevertheless the important structural diversity as well as their complexity make the sourcing of specific enzymes difficult.Two...
Tipo: Text Palavras-chave: Bactéries marines; Bactériophages marins; Phages marins; Exopolysaccharide; Marine bacteria; Marine bacteriophages; Marine phages; Polysaccharidase; Cazyme.
Ano: 2014 URL: https://archimer.ifremer.fr/doc/00498/60944/64337.pdf
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Viral degradation of marine bacterial exopolysaccharides ArchiMer
Lelchat, Florian; Mocaer, P Y; Ojima, T; Michel, G; Sarthou, Geraldine; Bucciarelli, Eva; Cérantola, S; Colliec-jouault, Sylvia; Boisset, Claire; Baudoux, A-c.
The identification of the mechanisms by which marine dissolved organic matter (DOM) is produced and regenerated is critical to develop robust prediction of ocean carbon cycling. Polysaccharides represent one of the main constituents of marine DOM and their degradation is mainly attributed to polysaccharidases derived from bacteria. Here, we report that marine viruses can depolymerize the exopolysaccharides (EPS) excreted by their hosts using 5 bacteriophages that infect the notable EPS producer, Cobetia marina DSMZ 4741. Degradation monitorings as assessed by gel electrophoresis and size exclusion chromatography showed that 4 out of 5 phages carry structural enzymes that depolymerize purified solution of Cobetia marina EPS. The depolymerization patterns...
Tipo: Text Palavras-chave: Marine phage; EPS; Polysaccharidase; DOM; Ocean.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00499/61062/64512.pdf
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